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DOI :
10.3791/57651-v
June 13th, 2018
Chapters
0:04
Title
0:53
Preparation of Neutralized Collagen
1:55
Sample Preparation for Light-sheet Fluorescence Microscopy Using Capillaries
4:08
Image Acquisition Using Light-sheet Microscopy
5:07
Fixation and Immunofluorescence Staining of Cells in Collagen Matrices
7:17
Results: Visualization of Human CTL Cells by Light-sheet Microscopy
8:32
Conclusion
ここでは、光シート顕微鏡を用いた三次元 (3 D) コラーゲン マトリックスに埋め込まれた免疫細胞を可視化するためのプロトコルを提案する.このプロトコルはまた、3 D の細胞の移動を追跡する方法を詳しく説明します。3 D マトリックスの懸濁細胞の他のタイプのこのプロトコルを用いることができます。
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