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DOI :
10.3791/57674-v
May 3rd, 2018
Chapters
0:04
Title
1:25
Prediction of Cdk1-specific Phosphorylation Sites and Expression of CENP-F in Escherichia coli
4:11
Recombitant Protein Purification by Glutathione-affinity Chromatography and Gel Filtration Followed by In Vitro Kinase Assay with Cdk1/Cyclin B
9:50
Results: Use of In Vitro Kinase Assay to Study Cdk1-specific Phosphorylation Sites in CENP-F
11:13
Conclusion
细胞周期蛋白依赖性激酶 1 (Cdk1) 在 G2 阶段被激活并且调控许多细胞通路。在这里, 我们提出了一个协议的体外激酶检测与 Cdk1, 这使得识别 Cdk1-specific 磷酸化的网站, 以建立细胞目标的这个重要的激酶。
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