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DOI :
10.3791/57674-v
May 3rd, 2018
Chapters
0:04
Title
1:25
Prediction of Cdk1-specific Phosphorylation Sites and Expression of CENP-F in Escherichia coli
4:11
Recombitant Protein Purification by Glutathione-affinity Chromatography and Gel Filtration Followed by In Vitro Kinase Assay with Cdk1/Cyclin B
9:50
Results: Use of In Vitro Kinase Assay to Study Cdk1-specific Phosphorylation Sites in CENP-F
11:13
Conclusion
サイクリン依存性キナーゼ 1 (Cdk1) 細胞周期 G2 段階で活性化し、多くの細胞経路を調節します。この重要なキナーゼの細胞内標的を確立するため Cdk1 固有のリン酸化部位の同定を可能にする Cdk1 と体外キナーゼ試金のためのプロトコルを紹介します。
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