Differentiation of hPSCs into Cryopreservable First Heart Field-like Cardiac Progenitors (FHF-L CPs)
4:38
FHF-L CP Differentiation into Ventricular-like Cardiomyocytes
6:28
Use of Activin A and Lentivirus-mediated Id1 Overexpression to Generate Ventricular-like Cardiomyocytes
8:12
Conclusion
Transcript
The main advantage of this simple technique is to generate cardiac progenitors and cardiomyocytes of defined heart field origin and subtypes. In turn, resulting cells can be used for a wide range of research application, including basic developmen
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在这里, 我们描述了一种可伸缩的方法, 使用激活 a 和慢病毒北疆介导的 Id1-overexpression 的简单组合, 从人类多潜能干细胞中生成第一个心场样的心脏祖细胞和心室样心肌细胞。