Infection and Image Acquisition of Local Ca2 Responses
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Results: Calcium Imaging During Shigella Infection
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Conclusion
Transcript
The overall goal of this fluorescent microscopy analysis using chemical probes is to analyze local and global calcium responses elicited by host cells during infection by pathogenic Shigella. This method can help answer key questions in the field
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Vi præsenterer her, protokoller for at visualisere calcium (Ca2 +) svar fremkaldes ved HeLa celler inficeret af Shigella. Ved at optimere parametre af bakteriel infektion og imaging med Ca2 + fluorescerende sonder, er atypiske globale og lokale Ca2 + signaler fremkaldt af bakterier over en lang række infektion kinetik karakteriseret.