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Developmental Biology

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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

9.8K Views

06:17 min

August 25th, 2018

August 25th, 2018

9,840 Views

0:00

Title

0:20

Skin Tissue Pretreatment

1:07

Digestion of skin tissue

2:11

Collecting and culturing primary cells

3:42

Cell Passage

4:46

Representative results

6:06

Conclusion

Transcript

The general goal of this video is to introduce a new simple method to isolate and culture primary human epidermal cells from an adult skin tissue. This advanced method is more suitable for producing a large number of high-potential epidermal cells

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Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells.

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