En roman mætning mutagenese tilgang: Trinvist karakterisering af regulerende Protein bindende websteder i RNA ved hjælp af Phosphorothioates

0:30

Generation of a Library of Mutants - Doping DNA Template with Non-wild Type Nucleotides

1:20

Synthesis of RNA

5:24

Protein Binding Reaction and Separation of Bound RNA

8:05

Analysis of Iodine-cleaved Phosphorothioate Products for Detection of Mutant Nucleotide Positions

9:07

Results: Characterization of Protein Binding Site in RNA

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Conclusion