Bir roman doygunluk Mutagenesis yaklaşım: Tek adım karakterizasyonu düzenleyici protein RNA Phosphorothioates kullanarak siteleri bağlama

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Generation of a Library of Mutants - Doping DNA Template with Non-wild Type Nucleotides

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Synthesis of RNA

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Protein Binding Reaction and Separation of Bound RNA

8:05

Analysis of Iodine-cleaved Phosphorothioate Products for Detection of Mutant Nucleotide Positions

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Results: Characterization of Protein Binding Site in RNA

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Conclusion