Cloning the Recipient Vector to Include a Tissue-specific Promoter
3:48
Cloning the Sponge
4:55
Generation of Stable H9c2 miR-181-sponge Expressing Cells
5:53
Synthesis and Purification of Sponge-nanoparticles (miR-181-sponge Nanoparticles)
7:01
Results: Validation of Nanonvector Delivery of the miR-181-sponge and its Effect on miR-181c Expression
7:58
Conclusion
Transcript
So for the last couple of decades, the importance of microRNA has been recognized in human disease conditions, but the problem is that, therapy-wise, it did not go to the primetime. One of the problem is, one microRNA can have different targets in
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Gewebe-spezifische MicroRNA-Hemmung ist eine Technologie, die im Feld MicroRNA unterentwickelt ist. Hier beschreiben wir ein Protokoll, um erfolgreich die MiR-181 MicroRNA-Familie in Myoblast Zellen aus dem Herzen zu hemmen. Nanovector-Technologie wird verwendet, um eine MicroRNA Schwamm zu liefern, die erhebliche in Vivo Cardio-spezifische MiR-181 Familie Hemmung zeigt.