Cloning the Recipient Vector to Include a Tissue-specific Promoter
3:48
Cloning the Sponge
4:55
Generation of Stable H9c2 miR-181-sponge Expressing Cells
5:53
Synthesis and Purification of Sponge-nanoparticles (miR-181-sponge Nanoparticles)
7:01
Results: Validation of Nanonvector Delivery of the miR-181-sponge and its Effect on miR-181c Expression
7:58
Conclusion
Transcript
So for the last couple of decades, the importance of microRNA has been recognized in human disease conditions, but the problem is that, therapy-wise, it did not go to the primetime. One of the problem is, one microRNA can have different targets in
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Inibição de microRNA tecido-específica é uma tecnologia que é subdesenvolvida no campo do microRNA. Aqui, descrevemos um protocolo para inibir com sucesso a família miR-181 microRNA nas células myoblast do coração. Nanovector tecnologia é usada para entregar um microRNA esponja que demonstra significativa na vivo cardio-específicos miR-181 família inibição.