Immunofluorescence Staining and Bromodeoxyuridine Labeling
10:05
Results: Distinct activation of neurogenesis in the DG in response to HFS in DG and PP
11:46
Conclusion
Transcript
The overall goal of this experiment is to observe the effect of high-frequency stimulation on neuronal activity in neurogenesis. This method can help answer key questions in an aligned cellular and molecular mechanisms of high-frequency deep brain
Sign in or start your free trial to access this content
Denne protokollen viser hvordan du setter opp en pålitelig HFS metode i mus. Nerveceller i hele den hippocampus dentate gyrus stimulert elektrisk av HFS direkte og indirekte i vivo. Neuronal aktivitet og molekylære signalene er undersøkt av c-fos og Notch1 immunofluorescent flekker, henholdsvis; neurogenesis er kvantifisert ved bromodeoxyuridine merking analysen.