Histotypic Tissue Culture

Overview

Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.

Procedure

Histotypic tissue culture allows cells to be grown in three dimensions, thereby creating in-vitro tissue morphologies that closely mimic realistic tissue function, which can be used as viable constructs for tissue repair. These cultures are typically three dimensional structures consisting of a single cell type grown in high density. The three dimensional structure, also known as the scaffold, mimics the natural extracellular matrix. Depending on the cell type used, scaffolds can be designed for a spec