This method can help answer key questions in the antimicrobial peptide field about whether peptides localize to or trans locate across the cell membrane. Using spheroplasts and protoplasts allows the visualization and analysis of peptide localizat
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Her præsenterer vi en protokol for at producere gram-negative Escherichia coli (E. coli) spheroplasts og gram-positive Bacillus megaterium (B. megaterium) protoplasts at visualisere, klart og hurtigt karakterisere peptid-bakterier interaktioner. Dette giver en systematisk metode til at definere membran lokalisering og translocating peptider.