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JoVE Journal

Neuroscience

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Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons

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07:43 min

January 7th, 2019

January 7th, 2019

6,861 Views

0:04

Title

0:36

Dissection

1:49

Spinal Cord Cell Suspension

2:58

Motoneuron Enrichment by Gradient Density

4:42

Motoneuron Culture and Magnetofection

6:00

Results: Confocal Images of Magnetofected Motoneurons

6:59

Conclusion

Transcript

This method can help to address key questions in neurobiology, such as motor neuron polarity, axon guidance, axon trafficking, and neurodegenerative mechanisms. The main advantage of this technique is to obtain a highly enriched motor neuron cultu

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The goal of this technique is to prepare a highly enriched culture of primary motoneurons (MNs) from murine spinal cord. To evaluate the consequences of mutations causing MN diseases, we describe here the isolation of these isolated MNs and their transfection by magnetofection.

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