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JoVE Journal

Immunology and Infection

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Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples

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06:59 min

June 30th, 2018

June 30th, 2018

7,243 Views

0:04

Title

0:44

Preparation for Virus Inactivation and Rapid Nucleic Acid Extraction

2:02

Blood Collection from Patients with Signs and Symptoms of a Virus Infection

2:50

Simplified Nucleic Acid Extraction from Whole Blood

5:04

Results: Sensitivity of the Simplified NA Extraction Method

6:22

Conclusion

Transcript

This method can help when performing point of care diagnostics, of patients with signs of viral infections. The main advantage of this technique is that it can be performed without laboratory equipment and electricity, and hence it can be performe

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Here, we present a protocol for the rapid virus nucleic acid extraction from the virus-inactivated whole blood. The extraction is performed directly in the blood collection tubes and requires no equipment or electricity. The method is not dependent on laboratory facilities and can be used anywhere (e.g., in field hospitals).

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