Generation and Maintenance of Astrocyte Progenitors (hAstros) from hPSCs
2:41
Generation and Maintenance of Neurons (hNeurons) and Inducible Neurons (iNeurons) from hPSCs
3:27
Preparation and Maintenance of 3D Sphere Co-cultures
5:42
Measurement of Live Synaptic Physiology with Multielectrode Arrays (MEAs)
6:56
Results: Morphological and Physiological Characteristics of Synaptic Microcircuits
8:07
Conclusion
Transcript
This method can help answer key questions in neuroscience regarding how intercellular communication between multiple human neural cell types contributes to synaptic circuit formation and function. This technique rapidly and systematically generate
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In this protocol, we aim to describe a reproducible method for combining dissociated human pluripotent stem cell derived neurons and astrocytes together into 3D sphere cocultures, maintaining these spheres in free floating conditions, and subsequently measuring synaptic circuit activity of the spheres with immunoanalysis and multielectrode array recordings.