Naïve (CD25-CD44loCD62Lhi) CD4+ T Cell Fluorescence-activated Cell Sorting (FACS) and Granulocyte Macrophage-colony-stimulating Factor (GM-CSF) T Helper (THGM) Cell Differentiation
5:48
Mouse THGM Cell Analysis
7:35
Results: THGM Cytokine Profile Analysis
9:30
Conclusion
Transcript
This method can help answer key questions in the CD4 T-cell field such as which subset of T-helper cells is the major source of GM-CSF in pathogenesis of the inflammatory diseases such as multiple sclerosis. The main advantage of this technique is
Sign in or start your free trial to access this content
Here, we present a protocol to differentiate murine granulocyte-macrophage-colony-stimulating-factor-producing T helper (THGM) cells from naive CD4+ T cells, including isolation of naive CD4+ T cells, differentiation of THGM, and analysis of differentiated THGM cells. This method can be applied to studies of the regulation and function of THGM cells.