Naïve (CD25-CD44loCD62Lhi) CD4+ T Cell Fluorescence-activated Cell Sorting (FACS) and Granulocyte Macrophage-colony-stimulating Factor (GM-CSF) T Helper (THGM) Cell Differentiation
5:48
Mouse THGM Cell Analysis
7:35
Results: THGM Cytokine Profile Analysis
9:30
Conclusion
Transcript
This method can help answer key questions in the CD4 T-cell field such as which subset of T-helper cells is the major source of GM-CSF in pathogenesis of the inflammatory diseases such as multiple sclerosis. The main advantage of this technique is
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Qui, presentiamo un protocollo per differenziare murino granulocyte-macrophage-colony-stimulating-factor-producing linfociti T helper (THGM) da cellule T CD4 + ingenuo, incluso l'isolamento delle cellule T CD4 + Naive, differenziazione di THGM, e analisi delle cellule di THGM differenziate. Questo metodo può essere applicato agli studi del regolamento e la funzione delle cellule di THGM.