JoVE Logo
Faculty Resource Center

Sign In

An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

DOI :

10.3791/58118-v

August 22nd, 2018

August 22nd, 2018

18,788 Views

1LamImSys Lab, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 2LamImSys Lab, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 3CIBER de Enfermedades Cardiovasculares

Here, we present a protocol for the in vivo determination of naïve CD4 T cell (T cell) activation, proliferation, and Th1 differentiation induced by GM-CSF bone marrow (BM)-derived dendritic cells (DCs). In addition, this protocol describes BM and T-cell isolation, DC generation, and DC and T-cell adoptive transfer.

-- Views

Related Videos

article

Retroviral Transduction of T-cell Receptors in Mouse T-cells

article

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

article

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

article

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

article

Bone Marrow-derived Macrophage Production

article

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

article

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes

article

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

article

T Cells Capture Bacteria by Transinfection from Dendritic Cells

article

Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved