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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

DOI :

10.3791/58130-v

14:48 min

August 25th, 2018

August 25th, 2018

24,303 Views

1Allen Institute for Cell Science

Described here is a protocol for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.

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Endogenous Protein Tagging

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