Quantifying Efferocytic Uptake and Dynamics Using a Fixed Cell Efferocytosis Assay and Inside-out Staining
5:14
Results: Overnight Culture of Jurkat Cells with Staurosporine Results in Apoptosis of Greater than 95% of Cells
5:36
Conclusion
Transcript
This method can be used to answer key questions in the efferocytosis field such as the role of signaling molecules in mediating the uptake of apoptotic cells. The main advantage of this technique is that it provides a clear quantification of apopt
Sign in or start your free trial to access this content
Efferocytosis, fagocyterende fjernelse af apoptotiske celler, er forpligtet til at opretholde homøostase og lettes ved receptorer og signalering veje, der giver mulighed for anerkendelse, engulfment og internalisering af apoptotiske celler. Heri, præsenterer vi en Fluorescens mikroskopi protokol til kvantificering af efferocytosis og aktivitet af efferocytic signaling veje.