Quantifying Efferocytic Uptake and Dynamics Using a Fixed Cell Efferocytosis Assay and Inside-out Staining
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Results: Overnight Culture of Jurkat Cells with Staurosporine Results in Apoptosis of Greater than 95% of Cells
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Conclusion
Transcript
This method can be used to answer key questions in the efferocytosis field such as the role of signaling molecules in mediating the uptake of apoptotic cells. The main advantage of this technique is that it provides a clear quantification of apopt
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Efferocytosis, de fagocytische verwijdering van apoptotic cellen, is nodig om de homeostase te handhaven en wordt vergemakkelijkt door de receptoren en signaalroutes waarmee voor de erkenning, de engulfment, en de internalisering van de apoptotic cellen. Hierin presenteren wij een fluorescentie microscopie protocol voor de kwantificering van efferocytosis en de activiteit van efferocytic signaalroutes.