This method can help answer key questions in the NK cell biology field about the role and function of specific genes and pathways that regulate effector cell function. The main advantage of this technique is its high success rate compared to DNA-d
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Here, we present a protocol to genetically modify primary or expanded human natural killer (NK) cells using Cas9 Ribonucleoproteins (Cas9/RNPs). By using this protocol, we generated human NK cells deficient for transforming growth factor–b receptor 2 (TGFBR2) and hypoxanthine phosphoribosyltransferase 1 (HPRT1).