Tick Surface Sterilization, DNA Purification, and 16S rRNA Gene Amplification
2:30
16S Amplicon Purification
3:59
Sample Barcoding and Purification
4:58
Library Quantification and Normalization
6:01
Results: Community Ecology of the Ixodes Microbiome
6:43
Conclusion
Transcript
This method can help answer key questions in the field of vector-borne disease ecology such as, what role does the vector microbiome play in pathogen dynamics, and how do vector microbiomes form and vary? The main advantages of this technique are
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Her præsenterer vi en next-generation sequencing protokol til 16S rRNA sekvensering, som muliggør identifikation og karakterisering af mikrobielle samfund inden for vektorer. Denne metode involverer DNA udvinding, forstærkning og stregkodesystem prøver gennem PCR, sekventering på en flow-celle, og bioinformatik at matche sekvens data til fylogenetiske oplysninger.