General Lymantria dispar (LD652) Cell and Virus Culture
1:33
General Viral Infection Protocol for Luminescence-based VSV rescue Assays in LD652 Cells
2:43
Luciferase Assay
4:06
Results: Coinfection with VSV-LUC with VACV-WR Results in More LUC Signals
5:04
Conclusion
Transcript
This method can help answer key questions in the field of viral immunology, such as, which host factors restrict arbovirus replication, and, in turn, which virus-encoded immune evasion proteins overcome these host restriction mechanisms. The main
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Vi præsenterer her, protokoller for at identificere 1) virus-kodet hæmmende, der fremmer arbovirus replikering og 2) eukaryote vært faktorer, der begrænser arbovirus replikation. Disse fluorescens - og luminescens-baserede metoder giver forskere hurtigt få kvantitative udlæsninger af arbovirus replikering i forsimplede assays med lavt signal-støj-forhold.