General Lymantria dispar (LD652) Cell and Virus Culture
1:33
General Viral Infection Protocol for Luminescence-based VSV rescue Assays in LD652 Cells
2:43
Luciferase Assay
4:06
Results: Coinfection with VSV-LUC with VACV-WR Results in More LUC Signals
5:04
Conclusion
Transcript
This method can help answer key questions in the field of viral immunology, such as, which host factors restrict arbovirus replication, and, in turn, which virus-encoded immune evasion proteins overcome these host restriction mechanisms. The main
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Her presenterer vi protokoller for å identifisere 1) virus-kodede immunomodulators som fremmer arbovirus replikering og 2) eukaryote vert faktorer som begrenser arbovirus replikering. Metodene fluorescens og luminescence-baserte tillate forskere å raskt få kvantitative readouts arbovirus replikasjon i enkle analyser med lav signal-til-støy-forhold.