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JoVE Journal

Immunology and Infection

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Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning

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This method provides an alternative to conventional fluorescence labeling and flow cytometry analysis procedure, which are time-consuming, costly, and incur the risk of altering the cellular function of the samples. The main advantage of this tech

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We describe a protocol for the label-free identification of lymphocyte subtypes using quantitative phase imaging and a machine learning algorithm. Measurements of 3D refractive index tomograms of lymphocytes present 3D morphological and biochemical information for individual cells, which is then analyzed with a machine-learning algorithm for identification of cell types.

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