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DOI :
10.3791/58325-v
•
7:15 min
August 19th, 2018
Chapters
0:04
Title
1:10
Preparation of Concanavalin-A-coated Glass-bottomed Dishes and the Plating of S2R+ Cells
2:25
Efficacy Test of the Fog-conditioned Medium and the Cellular Contractility Assay
3:16
Fixing and Staining of Constricted S2R+ Cells
5:18
Imaging and Quantification of the Cellular Contractility Assay
5:41
Results: Post-treatment with Fog-Myc, RLC Forms Rings Indicative of Non-Muscle Myosin II Constriction
6:40
Conclusion
ここで我々 はショウジョウバエS2R + 細胞を用いた収縮アッセイをについて説明します。外因性リガンド、折原腸陥入 (霧) のアプリケーションは、シグナル伝達経路と細胞の収縮性の霧の活性化に します。この試金を使用して、シグナル伝達経路霧の中で細胞の収縮性蛋白質の規制を調査できます。
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