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Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

DOI :

10.3791/58339-v

October 22nd, 2018

October 22nd, 2018

10,901 Views

1Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics, Washington University School of Medicine, St. Louis, Missouri

An imaging-based method is described that can be used to identify S-phase and analyze cell cycle dynamics in the C. elegans hermaphrodite germline using the thymidine analog EdU. This method requires no transgenes and is compatible with immunofluorescent staining.

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