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JoVE Journal

Biochemistry

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Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

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08:12 min

September 28th, 2018

September 28th, 2018

12,651 Views

0:04

Title

0:35

Preparing the Blue Native (BN) Gel

3:09

Thylakoid Solubilization

4:19

BN-PAGE

5:05

2D-SDS-PAGE

6:18

Results: Protein Complex Pattern and the Composition of Thylakoid Protein Complexes Solubilized with β-DM and Digitonin

7:25

Conclusion

Transcript

This method can help answer key questions about the composition and dynamic organization of the photosynthetic protein complexes in plant thylakoid membrane. The main advantage of this technique is that it allows the analysis of functional interac

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A protocol for the elucidation of plant thylakoid protein complex organization and composition with blue native polyacrylamide gel electrophoresis (BN-PAGE) and 2D-SDS-PAGE is described. The protocol is optimized for Arabidopsis thaliana, but can be used for other plant species with minor modifications.

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