Preparation of Software for Dissection and Recording
2:05
Dissect and Prepare the Larval Drosophila CNS
3:35
Extracellular Recording of Drosophila CNS
5:22
Results: Neurophysiological Recordings from the CNS of Third-Instar Larvae of D. melanogaster
5:59
Conclusion
Transcript
This method can help answer key questions in the fields of insect toxicology and insect physiology by measuring the electrogenesis of the Drosophila melanogaster central nervous system. This allows one to test a wide range of scientific hypotheses
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This protocol describes a method to record the descending electrical activity of the Drosophila melanogaster central nervous system to enable the cost-efficient and convenient testing of pharmacological agents, genetic mutations of neural proteins, and/or the role of unexplored physiological pathways.