Name: bio-inspired polydopamine surface modification of nanodiamonds and its reduction of silver nanoparticles
Uploaded: 2018-11-14T13:00:00
Description: Watch this Scientific Journal Video about Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles at JoVE.com

这项研究得到了国家科学基金会 (ccf 1814797) 和密苏里大学研究委员会、材料研究中心和密苏里科技大学艺术与科学学院的支持

(一)试剂的制备
注意: 使用前请阅读并理解所有相关材料安全数据表 (msds)。有些化学物质有毒易挥发。请遵循特殊的处理程序和储存要求。在实验过程中, 请使用个人防护设备, 如手套、安全眼镜和实验室涂层, 以避免潜在的危险。
<ol><li>Tris-HCl 缓冲液的制备<ol>
<li>在100毫升的去离子化 h2o 中溶解 30.29 g tris 粉末,确保粉末完全溶解, 然后将溶液转移到 250 ml-体积瓶中。</...

<ol>
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本文提供了一个详细的协议, 说明了自聚合 da 涂层对 nd 的表面功能化, 以及 pda 层上的 ag [(nh3)2]+还原到 agnps (图 3).该策略能够通过简单地改变 da 浓度来产生各种厚度的 pda 层。agnps 的大小也可以通过改变金属离子溶液的原始浓度来控制。图 1 a 中的 tem 图像显示了未涂布的 100 nm nds, 这些 nd 倾向于形成微簇和聚合。?...

纳米金刚石 (nds) 是一种新型的碳基材料, 近年来在各种应用中得到了广泛的关注 1,2。例如, nds 的高表面积为金属纳米粒子 (nps) 提供了出色的催化剂支持, 因为它们具有超化学稳定性和导热性 3。此外, nd 由于具有出色的生物相容性和非毒性4,5, 在生物成像、生物传感和药物输送方面发挥着重要作用。
为了有效地扩展它们的功能, 在 nds 表面结合功能物种 (如蛋白质、核酸和纳米粒子6) 是很有价值的。虽然在纯化过程中, 在 nd 表面上产生了多种功能基团 (如羟基、羧基、内酯等), 但由于每个官能团的密度较低, 功能基团的共轭产率仍然很低。活性化学组7。这将导致不稳定的 nd, 这往往会聚合, 从而限制进一步的应用程序8。
目前, 最常用的方法功能化 nd, 是共价共轭使用铜无点击化学 9, 共价键的肽核酸(pna)10, 和自组装 dna11。还提出了 nds 的非共价包装, 包括碳水化合物改性 bsa4和 hsa12涂层。然而, 由于这些方法耗时且效率低下, 因此最好能开发一种简单且普遍适用的方法来修改 nd 的表面。
多巴胺 (da)13, 被称为大脑中的天然神经递质, 被广泛用于粘附和功能化纳米粒子, 如金纳米颗粒 (aunps)14, fe 2o315,和 sio216.自聚合 pda 层丰富氨基和酚类, 可进一步用于直接减少金属纳米颗粒或在水溶液中容易固定含有硫醇胺的生物分子。这一简单的方法最近被秦氏等人应用于 nd 的功能化。和我们的实验室17,18, 虽然 da 衍生物被用于修改nds 通过点击化学在早期的研究19,20。
在这里, 我们描述了一种简单的基于 pda 的表面修正方法, 它有效地实现了 nd 的功能化。通过改变 da 的浓度, 我们可以控制 pda 层的厚度从几纳米到几十纳米。此外, 金属纳米颗粒直接减少并稳定在 pda 表面, 而不需要额外的有毒还原剂。银纳米粒子的大小取决于银 [(nh3)2]+ 的初始浓度.这种方法允许 pda 在 nd 表面上的良好控制沉积和 nd 共轭 agnps的合成,极大地扩展了 nd 作为催化剂载体、生物成像和生物传感器。

<table border="0" cellpadding="0" cellspacing="0" style="width:839px;" width="838">
	<colgroup>
		<col />
		<col />
		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Nanodiamond</td>
			<td style="width:121px;">FND Biotech, Inc.</td>
			<td style="width:136px;">brFND-100</td>
			<td style="width:365px;">dispersed in water, and used without further purification</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Dopamine hydrochloride</td>
			<td style="width:121px;">Sigma</td>
			<td style="width:136px;">H8502-25G</td>
			<td>prepare freshly</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Silver Nitrate</td>
			<td style="width:121px;">Fisher</td>
			<td style="width:136px;">S181-25</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ammonium Hydroxide</td>
			<td style="width:121px;">Fisher</td>
			<td style="width:136px;">A669S-500</td>
			<td>highly toxic</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Tris Hydrochloride</td>
			<td style="width:121px;">Fisher</td>
			<td style="width:136px;">BP153-500</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">TEM grid carbon film</td>
			<td style="width:121px;">Ted Pella</td>
			<td style="width:136px;">01843-F</td>
			<td>300 mesh copper</td>
		</tr>
	</tbody>
</table>

bio-inspired polydopamine surface modification of nanodiamonds and its reduction of silver nanoparticles

由于 nd 表面功能基团的多样性, 纳米粒子 (nds) 的表面功能化仍然具有挑战性。在这里, 我们展示了一个简单的协议, 多功能表面改性的 nds 使用肌肉启发多巴胺 (pda) 涂层。此外, pda 在 nd 上的功能层可作为合成和稳定金属纳米颗粒的还原剂。如果将 nd 和多巴胺简单地混合在一起, 多巴胺 (da) 可以在 nd 表面自行聚合并自发形成多巴胺层。pda 层的厚度通过改变 da 浓度来控制。典型结果表明, 将 da 悬浮液加入 50 ~ 100μg/ml 到 100 nm nd 悬浮液可以达到 pda 层的厚度 ~ 5 ~ 15 nm。此外, pda-nd 被用作基材, 以减少金属离子, 如银 [(nh3)2]+, 以银纳米粒子 (agnps).agnps 的大小取决于银 [(nh3)2]+ 的初始浓度。随着 ag [(nh3)2] + 浓度的增加, nps 的数量增加, 以及 nps 的直径增加。总之, 这项研究不仅提出了一种简单的方法来修改 nd 的表面与 pda, 而且还演示了通过锚定各种感兴趣的物种 (如 agnps) 的先进应用的 nd 的增强功能。

用透射电镜分析了 nd 表面 pda 层的形成 (图 1)。由于较高浓度的 da 导致 pda 层较厚, 观察到不同厚度的 pda 层。此外, 在封装反应后, nds 溶液的颜色从无色变为深色, 而 da 的初始浓度越高, 溶液变得越深。
图 2描述了在100纳米 pda-nd 表面上将ag [(nh3)2] +还原到 agnp...

Watch this Scientific Journal Video about Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles at JoVE.com

Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles

纳米金刚石的生物激发多巴胺表面改性及其对银纳米粒子的还原

提出了一种用多巴胺对纳米十二烷表面进行功能化的简便协议。

Surface functionalization of nanodiamonds (NDs) is still challenging due to the diversity of functional groups on the ND surfaces. Here, we demonstrate a ...

该方法有助于回答纳米金刚石有效表面功能化的关键问题，在材料科学和生物医学领域有着广泛的应用。该方法可用于纳米金元。它也可以用于其他材料，如金属纳米粒子、磁性纳米粒子或需要活性生物聚合物涂层的表面。在这种方法中，纳米金蒙德用一种通用粘合剂多多聚胺涂层进行真菌化。通过改变多巴胺的浓度，可以很好地控制PDA层的厚度。首先，在100微升去水中溶解30.29克三分HCl粉末。将溶液转移到 250 毫升体积的烧瓶中。用去维化的水将烧瓶填充到生产线上，并混合以获得一个摩尔三叶HCl缓冲液。从该库存溶液中，通过串行稀释准备 20 毫升 0.1 摩尔三叶HCl缓冲液。使用 pH 米监测缓冲液时，使用一个摩尔盐酸将 pH 值调整为 8.5。接下来，用pH 8.5三分缓冲液将每毫升悬浮液1毫克的0.02毫升稀释至1毫升。搅拌混合物10分钟，获得每毫升0.02毫克纳米金微悬浮液。然后，将20毫克多巴胺盐酸溶解在两毫升pH 8.5三酯缓冲液中，通过涡旋30秒获得每毫升多巴胺盐酸溶液缓冲10毫克。将新鲜准备好的多巴胺溶液的 5、7.5 或 10 微升添加到纳米金刚石溶液中，具体取决于最终多巴胺盐酸浓度是 50、75 或 100 毫克/毫升。调整反应量后，在25摄氏度下大力搅拌混合物，在黑暗中搅拌12小时。然后，将多多胺涂层纳米金mond的悬浮液转移到1.5毫升离心管中，以1.6万克离心两小时。取出上一杯，每次用1毫升的去维化水洗三次，每次一小时。然后将200微升的去维化水加入洗涤的固体中，对混合物进行30秒的声波化，以重新消毒多巴胺涂层的纳米金刚石。连续稀释40微升的悬浮多多胺涂层纳米金mond两次与去ion水。然后，通过涡流将100毫克硝酸银溶解在10毫升的去维化水中。在烟气罩中，在硝酸银溶液中加入一个摩尔水氨，滴落，直到形成黄色沉淀物，定期摇动溶液。继续添加氨，直到沉淀物消失，以获得二胺银氢氧化液溶液。立即将二胺银溶液的4.3或6.4微升加入40微升稀释的纳米金微分分散体，最终浓度分别为每毫升0.4或0.6毫克。在此之后，将体积调整为 100 微升与去维水。使混合物声波化10分钟。然后，在16，000g下离心15分钟，去除自由银离子。丢弃上经，洗涤银纳米颗粒装饰多多胺涂层纳米金mond，将它们离心三次，在100微升分部脱水，每次5分钟。在银纳米粒子装饰的纳米金微钻石中加入100微升的去维水，通过声波重新消毒30秒。使用 250 到 550 纳米的 UV-Vis 光谱扫描来描述纳米金微。接下来，将五个微升的银纳米粒子装饰纳米金微星沉积在等离子清洁的碳涂层铜网格上，让它们坐三分钟。然后，用滤纸去除多余的溶液。通过涂抹一滴去电化水，让其坐15秒，然后用滤纸将其吸走，将每个网格洗涤三次。在使用透射电子显微镜对样品进行可视化之前，让栅格风干。未涂覆的纳米金mond往往形成微团和聚合体，而多多胺涂层的纳米金mond则形成良好的分散体。多巴胺浓度越高，纳米金微面就形成了较厚的多多聚胺层。未涂覆的纳米金mond分散是清晰无色的。在用五纳米厚的多聚胺层涂上纳米金微层后，分散物出现多云和棕色。随着较厚的多多聚胺涂层，分散外观逐渐变暗。当二胺银氢氧化物浓度为每毫升0.4至0.6毫克时，涂有15纳米厚多多胺层的纳米金上的二胺银的减少最成功。在较低浓度下形成的纳米金微太小，无法有效地研究。最大吸收度值表明，每毫升溶液0.4至0.6毫克形成的纳米粒子的直径分别约为20和30纳米。TEM显示，从每毫升0.4毫克二胺银溶液产生的银纳米颗粒宽约24纳米，而从每毫升溶液0.6毫克产生的纳米粒子宽约28纳米宽。在二胺银浓度较高时，纳米金微面上的纳米粒子数量也更大。使用本程序后，形成具有可控PDA厚度的分散的纳米金微。这项技术为研究人员探索纳米金微在催化剂、生物传感器和纳米载体方面的应用铺平了道路。无需使用任何额外的还原剂，PDA 辅助物化过程可以在金属前体减少时诱导银纳米粒子的形成，并在 PDA 涂层表面上固定它们。此外，PDA层包含开放功能组，可进一步用于结合序列和胺改性生物分子。

Research

JoVE Journal

Chemistry

A Method to Manipulate Surface Tension of a Liquid Metal via Surface Oxidation and Reduction

方法通过表面氧化还原操纵液态金属的表面张力

Controlling interfacial tension is an effective method for manipulating the shape, position, and flow of fluids at sub-millimeter length scales, where ...

科研

化学

Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers

微流控干纺及再生丝素纤维的表征

The protocol demonstrates a method for mimicking the spinning process of silkworm. In the native spinning process, the contracting spinning duct enables ...

Synthesis of Platinum-nickel Nanowires and Optimization for Oxygen Reduction Performance

铂镍纳米线的合成及减氧性能优化

Platinum-nickel (Pt-Ni) nanowires were developed as fuel cell electrocatalysts, and were optimized for the performance and durability in the oxygen ...

Synthesis and Performance Characterizations of Transition Metal Single Atom Catalyst for Electrochemical CO2 Reduction

Synthesis and Performance Characterizations of Transition Metal Single Atom Catalyst for Electrochemical CO2 Reduction

电化学 CO2还原的过渡金属单原子催化剂的合成及性能表征

This protocol presents both the synthesis method of the Ni single atom catalyst, and the electrochemical testing of its catalytic activity and selectivity ...

Ligand-Mediated Nucleation and Growth of Palladium Metal Nanoparticles

配体介导成核与钯金属纳米粒子的生长

The size, size distribution and stability of colloidal nanoparticles are greatly affected by the presence of capping ligands. Despite the key contribution ...

Surface Properties of Synthesized Nanoporous Carbon and Silica Matrices

合成纳米碳和二氧化硅矩阵的表面性能

In this work, we report the synthesis and characterization of ordered nanoporous carbon material (also called ordered mesoporous carbon material [OMC]) ...

Synthesis and Characterization of Amphiphilic Gold Nanoparticles

两栖金纳米粒子的合成与表征

Gold nanoparticles covered with a mixture of 1-octanethiol (OT) and 11-mercapto-1-undecane sulfonic acid (MUS) have been extensively studied because of ...

Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride

薄膜银/银氯化银电极的制造，具有精细控制的单层氯化银

This paper aims to present a protocol to form smooth and well-controlled films of silver/silver chloride (Ag/AgCl) with designated coverage on top of thin ...