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JoVE Journal

Neuroscience

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Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

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08:30 min

January 18th, 2019

January 18th, 2019

13,644 Views

0:04

Title

0:39

Preparation of Micropipettes and Agar Block

1:30

Spinal Cord Dissection and Slicing

3:46

Whole-cell Patch-clamp Recordings

6:57

Results: Voltage-clamp Recordings in SG Neurons

8:03

Conclusion

Transcript

This method can help clarify the spinal mechanisms underlying sensory transmission, nociceptive regulation and chronic pain or ache development The main advantage of this technique is that it can permit ideal neuronal preservation and it can mimic

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Here, we describe the essential steps for whole-cell patch-clamp recordings made from substantia gelatinosa (SG) neurons in the in vitro spinal cord slice. This method allows the intrinsic membrane properties, synaptic transmission and morphological characterization of SG neurons to be studied.

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