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9.8K Views
•
06:28 min
May 2nd, 2019
DOI :
10.3791/58577-v
Chapters
0:04
Title
0:24
Cotton Genotype Planting for R. reniformis Resistance Evaluation and Nematode Inoculation
1:42
Root Sample Preparation
2:53
Vegetative Propagation for Recovered Plant Seed Production and R. reniformis Root Infection Evaluation
4:36
Results: Representative Variations in Reniform Nematode Infection Responses
5:54
Conclusion
Transcript
该方法有助于改性线虫抗棉品种的发育。通过确定其他棉花品种的耐药基因型和繁殖种群的评价。该技术的主要优点是,它为重新形成线虫筛选提供了一种简单、无损的方法。
首先将一个棉花球放在直径四厘米的底部,每个种子21个中心高度锥形塑料锅。用蒸汽巴氏杀菌的土壤混合物填充锅,从锅的顶部大约两厘米。将塑料支点放入每个锅中,指定要种植的基因型,并在每个锅中种植一种相应相应棉花基因型的种子。
在每个盆中填充额外的土壤,以覆盖种子,并放置在一个生长室设置为28摄氏度的恒定温度与16小时的
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Summary
本文提出了一种方案,用于快速无损地筛选棉花基因型,以增强表位线虫的抗性。该协议涉及目视检查线虫感染棉苗的根部,以确定感染反应。然后,从每株植物的植物芽繁殖,以恢复植物的种子生产。
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