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A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

DOI :

10.3791/58582-v

December 1st, 2018

December 1st, 2018

11,028 Views

1Institute for Biochemistry and Biology, Cell Membrane Biophysics Group, University of Potsdam

This protocol describes a fluorescence fluctuation spectroscopy-based approach to investigate interactions among proteins mediating cell-cell interactions, i.e. proteins localized in cell junctions, directly in living cells. We provide detailed guidelines on instrument calibration, data acquisition and analysis, including corrections to possible artefact sources.

Tags

Fluorescence Fluctuation Spectroscopy

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