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DOI :
10.3791/58618-v
April 28th, 2019
Chapters
0:04
Title
0:35
Yeast Preparation
2:02
Imaging
4:54
Deconvolution and Bleach Correction
7:39
Movie Generation and Editing to Isolate and Analyze Individual Structures
10:09
Results: Representative 4-Dimensional (4D) Yeast Microscopy
11:24
Conclusion
该协议描述了使用多色4D(延时3D)共聚焦显微镜对萌芽酵母中荧光标记细胞内腔的分析。选择成像参数是为了捕捉足够的信号,同时限制光损伤。自定义 ImageJ 插件允许跟踪和定量分析标记的结构。
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