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DOI :
10.3791/58618-v
April 28th, 2019
Chapters
0:04
Title
0:35
Yeast Preparation
2:02
Imaging
4:54
Deconvolution and Bleach Correction
7:39
Movie Generation and Editing to Isolate and Analyze Individual Structures
10:09
Results: Representative 4-Dimensional (4D) Yeast Microscopy
11:24
Conclusion
このプロトコルは、多色4D(タイムラプス3D)共焦点顕微鏡を用いた発芽酵母における蛍光標識細胞内コンパートメントの分析について説明する。イメージ投射パラメータは、光の損傷を制限しながら、適切な信号をキャプチャするために選択されます。カスタム ImageJ プラグインを使用すると、ラベル付き構造体を追跡し、定量的に分析できます。
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