Isolation of Apoptotic Cells and Apoptosis Reversal Procedure
8:22
Measurement of Breast CSC-like Cells by Flow Cytometry
9:11
Results: CSC-like Cells Transited from Breast Non-stem Cancer Cells During Apoptosis Reversal
10:29
Conclusion
Transcript
The isolations of genuine populations of apoptotic reversal cells has been a problem previously, which greatly limits our understanding on the consequences of apoptosis reversal. This protocol allow us to generate a purer populations of apoptotic
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Here, we present a protocol to isolate apoptotic breast cancer cells by fluorescence-activated cell sorting and further detect the transition of breast non-stem cancer cells to breast cancer stem cell-like cells after apoptosis reversal by flow cytometry.