Processing of Umbilical Cord Blood and the Isolation of Human HSPCs
4:25
Preparation of Human Hepatocytes for Transplantation
5:10
Preparation for the Surgery and Instrasplenic Transplantation of Human HSPCs and Hepatocytes in Mice
6:50
HIV Infection of TK-NOG Mice and Its Effect on the Human Liver and Immune System
7:48
Results: Analysis of the Dual Humanized TK-NOG Mouse Model
9:25
Conclusion
Transcript
The overall aim of the protocol is to generate a humanized mouse model with functional human immune system and liver using human hematopoietic stem cells and hepato site. This method can help answer key questions in genetic liver humanized mice. T
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This protocol provides a reliable method to establish humanized mice with both human immune system and liver cells. Dual reconstituted immunodeficient mice achieved via intrasplenic injection of human hepatocytes and CD34+ hematopoietic stem cells are susceptible to human immunodeficiency virus-1 infection and recapitulate liver damage as observed in HIV-infected patients.