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7.1K Views
•
07:01 min
January 11th, 2019
DOI :
10.3791/58663-v
Chapters
0:04
Title
0:44
Murine Neural Stem-Cell (NSC) Culture and Pre-Differentiation
2:02
Jet Acquisition
2:57
NSC Plasma Treatment and Differentiation
4:12
Immunofluorescence Staining
5:35
Results: Representative NSC Fate Specification
6:31
Conclusion
Transcript
这种方法有可能增强神经干细胞在体外分化,并有助于治疗神经疾病,如帕金森病。该技术的主要优点是,冷大气等离子体可以一步地用于组织移植神经干细胞的安全定向分化。冷大气血浆可以在短时间内增强神经干细胞分化。
并且需要更多的损伤细胞,提供所需的细胞移植。首先在25厘米方的烧瓶中播种约五倍十至第五个穆林神经干细胞。在37摄氏度和5%的二氧化碳下,含有5毫升完整的DMEM,为期两到三天。
当细胞达到85%的汇合时,用一毫升PBS洗涤培养。治疗前,用一毫升25%的尝试性药物一分钟
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Summary
该方案旨在提供神经干细胞冷大气等离子体处理和免疫荧光检测的详细实验步骤, 以提高分化。
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