January 7th, 2019
•In this protocol, we describe the complete workflow for rapid isolation of extracellular vesicles from human whole blood and characterization of specific markers by fluorescence-based nanoparticle-tracking analysis. The presented results show a high level of reproducibility and can be adjusted to cell culture supernatants.
Related Videos
A Fluorescence-based Assay of Phospholipid Scramblase Activity
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
Isolation of Tissue Extracellular Vesicles from the Liver
Imaging of Extracellular Vesicles by Atomic Force Microscopy
Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
Cryo-EM and Single-Particle Analysis with Scipion
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved