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DOI :
10.3791/58754-v
January 7th, 2019
Chapters
0:04
Title
0:34
Infection of HEK293 Mammalian Cells with P2 Baculovirus for Large-scale Protein Expression
2:42
Purification of hTRPC3 Protein from the Frozen Cell Pellet
5:26
Results: Structural Determination of hTRPC3
7:36
Conclusion
このプロトコルは、バキュロ ウイルス システムを効率的に最小限の労力と毒性、蛋白質の抽出、精製、哺乳類細胞の遺伝子を表現するために使用を含むクライオ電子顕微鏡によるイオン チャネルの構造を決定するために使用方法をについて説明します品質チェック、サンプル グリッドとスクリーニングと同様、データ収集と処理。
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