Preparing the 6-chambered Slides for the Flow Experiment
1:49
Fluidics Unit Preparation
3:07
Cell Loading
4:04
Attaching the Fluidics Unit and Imaging the Cells
5:28
Migration Track Analysis
7:02
Results: Quantifying Marginal Zone B-cell Shuttling
8:00
Conclusion
Transcript
This method can help understand key questions in the immune cell migration field, such as how the cytoskeleton sends shear forces and how integrin ligands and chemokines affect flow-induced migration of leukocytes. The main advantage of this techn
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Marginal zone B cells (MZBs) respond to the force of shear flow by re-orienting their migration path up the flow. This protocol shows how to record and analyze the migration using a fluidics unit, pump, microscope imaging system, and free software.