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JoVE Journal

Immunology and Infection

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Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

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08:38 min

February 22nd, 2019

February 22nd, 2019

5,594 Views

0:04

Title

1:08

Infection Procedures

2:16

Sampling Processing of Intracellular and Emergent Lm for Colony-forming Unit (CFU) Assay

3:58

Sampling Processing of Intracellular and Emergent Lm for Flow Cytometry (FCM)

6:22

Results: The Role of Pathogen and Host-encoded Factors on Cellular Infection

7:54

Conclusion

Transcript

Microbial Pathogens that replicate intracellularly must eventually exit the infected cell. Which, when compared to other aspects of host pathogen interactions is relatively understudied. Here, we describe techniques to analyze pathogen efflux.

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We describe both in vitro and in vivo infection assays that can be used to analyze the activities of host-encoding factors.

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