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DOI :
10.3791/58873-v
•
10:48 min
January 25th, 2019
Chapters
0:04
Title
0:39
Preparation of Photoactivatable Nicotine (PA-Nic)
3:23
Imaging Neurons with 2-Photon Laser Scanning Microscopy
5:28
Calibration of Uncaging Laser Galvanometers and Pairing of PA-Nic Laser Flash Photolysis with Electrophysiological Recordings
7:47
Results: Representative 2-Photon Laser Scanning Microscopy Imaging and PA-Nic Laser Flash Photolysis Responses
9:47
Conclusion
本文提出了一种用烟碱不加污染的方法研究小鼠脑片中烟碱乙酰胆碱受体 (nachr)。当与同时膜片钳记录和 2-光子激光扫描显微镜, 尼古丁发现连接烟碱受体功能与细胞形态, 提供了更深入的了解胆碱能神经生物学。
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