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42.2K Views
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10:16 min
March 12th, 2019
DOI :
10.3791/58879-v
Chapters
0:04
Title
0:46
Tissue Preparation by Cryosectioning
2:13
In vitro Autoradiography
4:44
Exposure to Phosphor Imaging Plates and Scanning
6:04
Densitometric Analysis of Digital Image
7:34
Results: Visualization and Characterization of Pharmacological Targets Using Radiography
9:34
Conclusion
Transcript
自动造影是一种强大而简单的技术,用于研究使用特定放射性基因在组织中蛋白质结合位点分布。它也是免疫基础化学的合适替代品。该技术通过比传统胶片曝光更快的数字图像,以空间分辨率可视化蛋白质表达。
它也可用于蛋白质靶点的药理分析。该方法涉及安装在玻璃上的小鼠组织,但可以扩展到其他物种,甚至扩展到在盖玻片上生长的细胞。演示程序将由纳内格里姆-克雷,一个博士生在我的实验室。
首先,将组织淹没在粉状干冰中,以捕捉冻结。将冷冻组织直接转移到温度设置为零下 20 摄氏度的低温恒温器。
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Summary
自体影像学方法通常用于研究放射线与组织切片的结合, 以确定定性或定量药理学。
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