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JoVE Journal

Neuroscience

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Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

5.2K Views

11:39 min

September 7th, 2019

September 7th, 2019

5,188 Views

0:05

Title

1:16

Preparation of Mouse Endothelial Culture in Permeable Support Inserts

1:55

Set-up of Neural-endothelial Co-culture and Ac4ManNAz Labeling System

3:25

Immunofluorescent Staining of Sialoglycoproteins in Expanded Primary NSPCs and Differentiated Neurons

5:25

Purification of Sialoglycoproteins from Expanded Primary NSPCs and Differentiated Neurons

8:45

Results: Identifying Cell Surface Markers by Metabolic Labeling of Sialoglycan

10:47

Conclusion

Transcript

Neural stem and progenitor cells can self-renew and differentiate into different neural cell types, supporting nervous system development and offering a resource for regenerative medicine. These cells are heterogeneous, and we need to know their s

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Presented here is a protocol that combines an in vitro neural-endothelial co-culture system and metabolic incorporation of sialoglycan with bioorthogonal functional groups to expand primary neural stem and progenitor cells and label their surface sialoglycoproteins for imaging or mass-spectrometry analysis of cell surface markers.

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