Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/58946-v
•
8:49 min
January 22nd, 2019
Chapters
0:04
Title
1:15
Kinase Inhibitor Library Screening (Centrifuge-independent Assay)
2:15
Stimulation of Thymocytes Using Anti-CD3/CD28 Beads
3:52
Staining of Surface Antigens and Fixation of Cells
5:09
Intracellular Staining for Active Caspase 3
6:05
Results: Flow-Cytometry-Based Screening of Modulators of T-cell Receptor Signaling and T-cell Activation
7:59
Conclusion
本稿は、化学剤のスクリーン ライブラリに阻害剤と T 細胞受容体のシグナル伝達に影響を与えるそれらのターゲットを識別するためフロー ベース フローサイトメトリー分析を使用します。ここで説明する方法は、ハイスループットス クリーニングにも拡張できます。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved