Name: a behavioral test battery for the repeated assessment of motor skills, mood, and cognition in mice
Uploaded: 2019-03-02T13:00:00
Description: Watch this Scientific Journal Video about A Behavioral Test Battery for the Repeated Assessment of Motor Skills, Mood, and Cognition in Mice at JoVE.com

作者感谢香港大学生物医学学院的 cora sw lai 博士提供了高架加迷宫测试, 并感谢香港大学麻醉学系提供了 rotarod 测试仪器。

这里所描述的所有方法都已获得香港大学教与研究中使用活体动物委员会 (culatr) 的批准。
1. 一般议定书
请注意:本节以执事19为基础。
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行为室设置
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<li>在行为室中, 消除不相关的刺激/干扰, 包括实验设备上的直射明亮的光线、气味、噪音和其他不相关的动物 (应在约10米2 , 可调节的照明, 最好...

<ol>
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M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=husbandry+and+handling+of+rodents+for+behavioral+experiments.">husbandry and handling of rodents for behavioral experiments.</a> Nature Protocols. 1 (2), 936-946 (2006).</li><li>Poon, D. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=PKR+deficiency+alters+E.+coli-induced+sickness+behaviors+but+does+not+exacerbate+neuroimmune+responses+or+bacterial+load.">PKR deficiency alters E. coli-induced sickness behaviors but does not exacerbate neuroimmune responses or bacterial load.</a> Journal of Neuroinflammation. 12, 212 (2015).</li><li>O'Leary, T. P., Gunn, R. K., Brown, R. 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小鼠行为分析对神经退行性变的研究至关重要。虽然认知功能往往是神经退行性疾病最容易受到影响的行为领域, 但抑郁和焦虑等情绪障碍往往是合意的。此外, 在一些测试中, 如新的目标识别试验、高架加迷宫测试和社会互动测试, 运动函数往往会影响对结果的解释。基于这些想法, 需要一个全面的行为测试电池, 以便对行为进行全面和准确的评估。
第一步是选择正确的测试?...

神经退行性疾病具有破坏性的行为症状, 包括认知障碍、焦虑和抑郁等情绪障碍或运动功能障碍1。各种神经退行性疾病的发病机制尚不清楚2。累积的研究表明, 遗传和环境因素都可能导致神经退行性疾病的发病机制。确定神经退行性变的危险因素需要行为分析。虽然每种类型的神经退行性疾病都有其特征性行为症状 (例如, 阿尔茨海默氏病 [ad] 的特点是认知障碍和帕金森病 [pd] 与运动功能障碍)。随着病情的发展, 患者表现出不同行为异常的合并症 3.例如, ad 患者在晚期第4、5 阶段出现情绪功能障碍症状。pd 患者可能会发展为与 pd 相关的痴呆, 并出现认知障碍6。基于这些特征, 神经退行性变模型的行为分析通常是全面和重复的。
为了实现这一目标, 设计了一种具有良好有效性的经典且广泛使用的行为测试的电池, 用于电机、情绪和认知方面的行为分析。通过开场试验7、8 和加速旋转试验, 可以对电机功能进行测试。情绪功能障碍, 包括社会功能障碍、抑郁和焦虑, 在神经退行性疾病中最常见5。因此, 这种电池包括社交性 9的社会互动测试、焦虑10的提升加迷宫测试和抑郁症的强制游泳测试。认知障碍是 ad 和额叶痴呆等神经退行性疾病中最典型的症状之一.认知领域, 包括短期记忆和插曲记忆, 容易发生神经退行性变13,14,15。因此, 电池中包括了空间学习和记忆16 的莫里斯水迷宫测试和短期记忆17的新目标识别测试。这些测试是相互兼容的。测试的顺序旨在最大限度地提高习惯和最大限度地减少干扰, 以进一步提高电池内的兼容性。由于每个函数至少由两个原则和方法不同的独立测试进行测试, 因此每个测试的结果可以进一步验证。此外, 还重点介绍了一些试验的方案, 以便反复检测, 从而促进神经退行性疾病发展的纵向研究。因此, 这种行为测试电池研究不同的子域的行为变化看到在不同阶段的神经退行变, 而花费的动物最少数量。这种电池已被用于一项纵向研究, 评估年轻成人 (3个月大) 男性 c57bl/6n 小鼠在呼吸接触二氧化硅纳米颗粒后的行为变化, 这是一种职业危害, 是一种潜在的危险因素。神经退行性变18。然而, 其他菌株或模型, 如衰老的小鼠和基因操纵的小鼠, 可能会表现不同于年轻的 c57bl/6n 小鼠。因此, 在这些鼠标中使用此电池时可能需要小心。

<table>
	<tbody>
		<tr>
			<td>chambers in social interaction test</td>
			<td>home made</td>
			<td></td>
			<td>(8 cm (L) x 6 cm (W) x 12 cm (H)), transparant with holes, plastic</td>
		</tr>
		<tr>
			<td>cylindrical tanks used in forced swimming test</td>
			<td>home made</td>
			<td></td>
			<td>30 cm height, 20 cm diameters, glass</td>
		</tr>
		<tr>
			<td>elevated plus maze</td>
			<td>home made</td>
			<td></td>
			<td>open arms (30 x 5 x 0.5 cm) ,closed arms (30 x 5 x 16 cm), center platform (5 x 5 x 0.5 cm), 40 cm tall. Plastic, nontransparant</td>
		</tr>
		<tr>
			<td>IITC Roto-Rod Apparatus</td>
			<td>IITC life science Inc.</td>
			<td>755, series 8</td>
			<td></td>
		</tr>
		<tr>
			<td>open field arena</td>
			<td>home made</td>
			<td></td>
			<td>60 cm (L) x 60 cm (W) x 40 cm (H), plastic, nontransparant</td>
		</tr>
		<tr>
			<td>water maze</td>
			<td>home made</td>
			<td></td>
			<td>120 cm in diameter, 60 cm deep, steel</td>
		</tr>
	</tbody>
</table>

a behavioral test battery for the repeated assessment of motor skills, mood, and cognition in mice

神经退行性变的药理和毒理研究需要对小鼠进行全面的行为分析, 因为运动功能障碍以及情绪和认知功能障碍是神经退行性疾病中常见且经常共有的症状。这里展示的是用于电机、情绪和认知的行为测试电池, 可以在纵向研究中反复测试。该电池通过至少两项独立的公认测试 (即运动功能的露场测试和 trogarod 测试、社会交往测试、升高加迷宫测试和情感功能的强制游泳测试, 莫里斯水迷宫测试和认知功能的新对象识别测试)。因此, 这种灵敏、全面的测试电池是研究神经退行性变行为变化的有力工具。

该行为测试电池是为全面有效的运动、情绪和认知行为分析而设计的, 这些运动、情绪和认知是神经退行性变5中常见的。我们应用该电池研究了年轻成人 c57bl/6n 小鼠在呼吸接触二氧化硅纳米颗粒1个月零18后的行为变化.结果表明, c57bl/6n 小鼠接触二氧化硅纳米颗粒后, 在不同暴露时间18 后表现出不同的行为变化。简单地?...

Watch this Scientific Journal Video about A Behavioral Test Battery for the Repeated Assessment of Motor Skills, Mood, and Cognition in Mice at JoVE.com

A Behavioral Test Battery for the Repeated Assessment of Motor Skills, Mood, and Cognition in Mice

一种用于小鼠运动技能、情绪和认知重复评估的行为测试电池

一个全面的行为测试电池的运动技能, 情绪-包括社会交往, 抑郁, 焦虑-和认知是设计的重复评估神经退化相关的行为变化在小鼠。

Pharmacological and toxicological studies in neurodegeneration require comprehensive behavioral analysis in mice because motor dysfunctions and ...

神经退行性疾病通常表现为破坏性行为症状，如认知障碍、情绪功能障碍，甚至运动功能障碍。这项研究允许对行为表型进行精确评估，具有高水平的可重复性。我们设计了一种全面的电池，可以反复用于小鼠的行为分析。本测试评估运动功能、社会互动、情感功能和认知功能。通过组织该电池的测试顺序和间隔，提高了测试效率，减少了测试之间的干扰和频繁操作对小鼠的压力。首先，按照文本协议中描述的顺序排列行为测试。在不透明、白色、塑料的竞技场中执行开放现场测试。开始摄像机录制，轻轻地将鼠标放在竞技场的墙中间，朝墙。记录鼠标的行为 10 分钟，然后返回到其主笼。接下来，在计算机屏幕上将竞技场分成四个四个方块。计算鼠标穿过竞技场中网格线以评估运动功能次数。要测量焦虑，测量鼠标在竞技场中心区域花费的时间量。在同一天进行高架加迷宫测试。一旦所有小鼠完成开放场测试，将鼠标放在张开和闭合臂的交界处。将鼠标的行为记录五分钟，然后再将鼠标返回到其家庭笼子。经过连续两天的开放现场测试，根据文本协议设置了新颖的对象识别测试。启动相机，将鼠标放在竞技场上，朝向墙的中间，熟悉鼠标。将鼠标的行为记录 10 分钟。然后把鼠标还到它的笼子里。熟悉后24小时，用一个新颖的物体重复测试。分别测量鼠标与新对象和熟悉对象交互的时间。然后计算鼠标的内存作为对新颖对象的首选项。对于社交互动测试，请使用具有两个透明室的开放式场地，表面有孔。将主题鼠标放在竞技场中，进行三分钟的物体探索，使鼠标进入房间。然后将主题鼠标返回到其家庭笼子并清洁竞技场。把一只新老鼠放进其中一个房间里。然后将主题鼠标重新引入竞技场并录制三分钟。在查看视频时，通过测量受攻击对象鼠标与新型鼠标室或空室交互的时间比例来测量社交互动。接下来执行加速旋转测试。在每次试验中，将鼠标放在面向机器对面的墙上的静态杆上。在出现可能的症状之前，在天真的小鼠中训练三天，使小鼠习惯到轮盘，使小鼠同样了解量表。当鼠标掉落时停止设备，并记录鼠标在杆上花费的时间。重复此过程两次，然后再将鼠标返回到其主笼。接下来，将水迷宫放在行为室的中心，并标记迷宫的位置，以确保水迷宫的位置保持不变。将迷宫划分为四个假想的象限。在每个象限的中心挂起视觉提示。然后在第四个象限的中心放置一个平台并标记其位置。用水填满迷宫，用奶粉染成白色，直到水位比平台高一厘米。每次训练试验开始时，开始录像，轻轻地把鼠标放在迷宫里。远离鼠标的视线。当鼠标在 60 秒内无法定位平台，或者鼠标在 60 秒内定位平台并在其上停留 10 秒时，将鼠标返回到其主笼。测试结束之后，停止视频，将鼠标放在其家庭笼中，在红外光下。最后执行如前所述的水迷宫测试的探针阶段，无需平台。接下来为强制游泳测试准备圆柱形坦克。开始录像，轻轻地将鼠标放在水箱中央的水中。将鼠标记录六分钟，然后将鼠标放回其家庭笼子。查看强制游泳测试的视频。测量视频最后五分钟的固定时间。该协议是研究小鼠在呼吸接触二氧化硅纳米粒子后的行为变化。公开场试验和加速轮盘试验结果表明，二氧化硅纳米粒子对小鼠的动车或运动功能没有影响。社会互动活动在接触二氧化硅纳米粒子一个月后受到影响，然而，开放场测试、高架加迷宫测试以及强迫游泳测试在暴露一个月后未显示任何焦虑或抑郁。两个月的二氧化硅纳米粒子接触确实会导致焦虑，升高加迷宫测试的结果就证明了这一点。在两个月的暴露后，在水迷宫测试中也检测到认知障碍。该电池设计用于C57小鼠应变神经退化的综合行为分析。它最适合神经退化相关的纵向研究，在神经毒素毒性或潜在的危险因素，或药物开发，往往具有长期的去药和反复测试。

Research

JoVE Journal

Behavior

The Resident-intruder Paradigm: A Standardized Test for Aggression, Violence and Social Stress

驻地入侵者范式：一种标准化考试的侵略，暴力和社会压力

This video publication explains in detail the experimental protocol of the resident-intruder paradigm in rats. This test is a standardized method to ...

科研

行为学

The Crossmodal Congruency Task as a Means to Obtain an Objective Behavioral Measure in the Rubber Hand Illusion Paradigm

一致性的任务作为一种手段来获取目标行为的措施中的橡胶手错觉范式Crossmodal

The rubber hand illusion (RHI) is a popular experimental paradigm. Participants view touch on an artificial rubber hand while the participants' own hidden ...

Automated, Quantitative Cognitive/Behavioral Screening of Mice: For Genetics, Pharmacology, Animal Cognition and Undergraduate Instruction

小鼠自动化，定量认知/行为筛选：对于遗传学，药理学，动物认知与本科教学

We describe a high-throughput, high-volume, fully automated, live-in 24/7 behavioral testing system for assessing the effects of genetic and ...

Compensatory Limb Use and Behavioral Assessment of Motor Skill Learning Following Sensorimotor Cortex Injury in a Mouse Model of Ischemic Stroke

补偿肢使用和运动技能学习行为评估以下感觉运动皮层损伤缺血性脑卒中的小鼠模型

Mouse models have become increasingly popular in the field of behavioral neuroscience, and specifically in studies of experimental stroke. As models ...

The Modified Hole Board - Measuring Behavior, Cognition and Social Interaction in Mice and Rats

修改后的孔板 - 测量行为，认知和社会互动小鼠和大鼠

This protocol describes the modified hole board (mHB), which combines features from a traditional hole board and open field and is designed to measure ...

The Double-H Maze: A Robust Behavioral Test for Learning and Memory in Rodents

双-H迷宫：一个强大的行为测试学习和记忆的鼠害

Spatial cognition research in rodents typically employs the use of maze tasks, whose attributes vary from one maze to the next. These tasks vary by their ...

Assessment of Social Cognition in Non-human Primates Using a Network of Computerized Automated Learning Device (ALDM) Test Systems

Assessment of Social Cognition in Non-human Primates Using a Network of Computerized Automated Learning Device ALDM Test Systems

社会认知的非人类灵长类动物评估使用电脑自动学习设备（ALDM）测试系统的网络

Fagot &#38; Paleressompoulle1 and Fagot &#38; Bonte2 have published an automated learning device (ALDM) for the study of cognitive abilities of monkeys ...

Assessment of Cocaine-induced Behavioral Sensitization and Conditioned Place Preference in Mice

可卡因诱导行为敏和小鼠位置偏爱评估

It is thought that rewarding experiences with drugs create strong contextual associations and encourage repeated intake. In turn, repeated exposures to ...

The Rodent Psychomotor Vigilance Test (rPVT): A Method for Assessing Neurobehavioral Performance in Rats and Mice

The Rodent Psychomotor Vigilance Test rPVT: A Method for Assessing Neurobehavioral Performance in Rats and Mice

鼠类精神运动警觉测试（rPVT）：一个在大鼠和小鼠的神经行为评估性能的方法

The human Psychomotor Vigilance Test (PVT) is a widely used procedure for measuring changes in fatigue and sustained attention. The present article ...

Assessing Working Memory in Children: The Comprehensive Assessment Battery for Children &#8211; Working Memory (CABC-WM)

Assessing Working Memory in Children: The Comprehensive Assessment Battery for Children ÃƒÆ’Ã‚Â¢ÃƒÂ¢Ã¢â‚¬Å¡Ã‚Â¬ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œ Working Memory CABC-WM

评估儿童工作记忆：儿童综合评估电池 - 工作记忆（CABC-WM）

The Comprehensive Assessment Battery for Children - Working Memory (CABC-WM) is a computer-based battery designed to assess different components of ...