Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
Results: Representative GC Aggregation Survival Before and After Antibiotic Treatment
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Conclusion
Transcript
This technique allows us to measure antibiotic susceptibility under more clinically relevant conditions. The technique also allows us to determine the true antibiotic concentration that can kill bacteria in aggregates. This technique can be applie
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A simple ATP-measuring assay and live/dead staining method were used to quantify and visualize Neisseria gonorrhoeae survival after treatment with ceftriaxone. This protocol can be extended to examine the antimicrobial effects of any antibiotic and can be used to define the minimal inhibitory concentration of antibiotics in bacterial biofilms.